A Molecular Chameleon for Mapping Subcellular Polarity in an Unfolded Proteome Environment.
Identifieur interne : 000923 ( Main/Exploration ); précédent : 000922; suivant : 000924A Molecular Chameleon for Mapping Subcellular Polarity in an Unfolded Proteome Environment.
Auteurs : Tze Cin Owyong [Australie] ; Pramod Subedi [Australie] ; Jieru Deng [Australie] ; Elizabeth Hinde [Australie] ; Jason J. Paxman [Australie] ; Jonathan M. White [Australie] ; Weisan Chen [Australie] ; Bego A Heras [Australie] ; Wallace W H. Wong [Australie] ; Yuning Hong [Australie]Source :
- Angewandte Chemie (International ed. in English) [ 1521-3773 ] ; 2019.
Abstract
Environmental polarity is an important factor that drives biomolecular interactions to regulate cell function. Herein, a general method of using the fluorogenic probe NTPAN-MI is reported to quantify the subcellular polarity change in response to protein unfolding. NTPAN-MI fluorescence is selectively activated upon labeling unfolded proteins with exposed thiols, thereby reporting on the extent of proteostasis. NTPAN-MI also reveals the collapse of the host proteome caused by influenza A virus infection. The emission profile of NTPAN-MI contains information of the local polarity of the unfolded proteome, which can be resolved through spectral phasor analysis. Under stress conditions that disrupt different checkpoints of protein quality control, distinct patterns of dielectric constant distribution in the cytoplasm can be observed. However, in the nucleus, the unfolded proteome was found to experience a more hydrophilic environment across all the stress conditions, indicating the central role of nucleus in the stress response process.
DOI: 10.1002/anie.201914263
PubMed: 31826303
Affiliations:
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type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Subedi, Pramod" sort="Subedi, Pramod" uniqKey="Subedi P" first="Pramod" last="Subedi">Pramod Subedi</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086</wicri:regionArea><wicri:noRegion>3086</wicri:noRegion></affiliation></author><author><name sortKey="Deng, Jieru" sort="Deng, Jieru" uniqKey="Deng J" first="Jieru" last="Deng">Jieru Deng</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086</wicri:regionArea><wicri:noRegion>3086</wicri:noRegion></affiliation></author><author><name sortKey="Hinde, Elizabeth" sort="Hinde, Elizabeth" uniqKey="Hinde E" first="Elizabeth" last="Hinde">Elizabeth Hinde</name><affiliation wicri:level="4"><nlm:affiliation>School of Physics, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne, Melbourne, VIC, 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>School of Physics, Department of Biochemistry and Molecular Biology, Bio21 Institute, The University of Melbourne, Melbourne, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Paxman, Jason J" sort="Paxman, Jason J" uniqKey="Paxman J" first="Jason J" last="Paxman">Jason J. Paxman</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086</wicri:regionArea><wicri:noRegion>3086</wicri:noRegion></affiliation></author><author><name sortKey="White, Jonathan M" sort="White, Jonathan M" uniqKey="White J" first="Jonathan M" last="White">Jonathan M. White</name><affiliation wicri:level="4"><nlm:affiliation>ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Parkville, VIC, 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Parkville, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Chen, Weisan" sort="Chen, Weisan" uniqKey="Chen W" first="Weisan" last="Chen">Weisan Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086</wicri:regionArea><wicri:noRegion>3086</wicri:noRegion></affiliation></author><author><name sortKey="Heras, Bego A" sort="Heras, Bego A" uniqKey="Heras B" first="Bego A" last="Heras">Bego A Heras</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086</wicri:regionArea><wicri:noRegion>3086</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Wallace W H" sort="Wong, Wallace W H" uniqKey="Wong W" first="Wallace W H" last="Wong">Wallace W H. Wong</name><affiliation wicri:level="4"><nlm:affiliation>ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Parkville, VIC, 3010, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>ARC Centre of Excellence in Exciton Science, School of Chemistry, Bio21 Institute, The University of Melbourne, Parkville, VIC, 3010</wicri:regionArea><orgName type="university">Université de Melbourne</orgName><placeName><settlement type="city">Melbourne</settlement><region type="état">Victoria (État)</region></placeName></affiliation></author><author><name sortKey="Hong, Yuning" sort="Hong, Yuning" uniqKey="Hong Y" first="Yuning" last="Hong">Yuning Hong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086, Australia.</nlm:affiliation><country xml:lang="fr">Australie</country><wicri:regionArea>Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, VIC, 3086</wicri:regionArea><wicri:noRegion>3086</wicri:noRegion></affiliation></author></analytic><series><title level="j">Angewandte Chemie (International ed. in English)</title><idno type="eISSN">1521-3773</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Environmental polarity is an important factor that drives biomolecular interactions to regulate cell function. Herein, a general method of using the fluorogenic probe NTPAN-MI is reported to quantify the subcellular polarity change in response to protein unfolding. NTPAN-MI fluorescence is selectively activated upon labeling unfolded proteins with exposed thiols, thereby reporting on the extent of proteostasis. NTPAN-MI also reveals the collapse of the host proteome caused by influenza A virus infection. The emission profile of NTPAN-MI contains information of the local polarity of the unfolded proteome, which can be resolved through spectral phasor analysis. Under stress conditions that disrupt different checkpoints of protein quality control, distinct patterns of dielectric constant distribution in the cytoplasm can be observed. However, in the nucleus, the unfolded proteome was found to experience a more hydrophilic environment across all the stress conditions, indicating the central role of nucleus in the stress response process.</div></front></TEI><affiliations><list><country><li>Australie</li></country><region><li>Victoria (État)</li></region><settlement><li>Melbourne</li></settlement><orgName><li>Université de Melbourne</li></orgName></list><tree><country name="Australie"><region name="Victoria (État)"><name sortKey="Owyong, Tze Cin" sort="Owyong, Tze Cin" uniqKey="Owyong T" first="Tze Cin" last="Owyong">Tze Cin Owyong</name></region><name sortKey="Chen, Weisan" sort="Chen, Weisan" uniqKey="Chen W" first="Weisan" last="Chen">Weisan Chen</name><name sortKey="Deng, Jieru" sort="Deng, Jieru" uniqKey="Deng J" first="Jieru" last="Deng">Jieru Deng</name><name sortKey="Heras, Bego A" sort="Heras, Bego A" uniqKey="Heras B" first="Bego A" last="Heras">Bego A Heras</name><name sortKey="Hinde, Elizabeth" sort="Hinde, Elizabeth" uniqKey="Hinde E" first="Elizabeth" last="Hinde">Elizabeth Hinde</name><name sortKey="Hong, Yuning" sort="Hong, Yuning" uniqKey="Hong Y" first="Yuning" last="Hong">Yuning Hong</name><name sortKey="Paxman, Jason J" sort="Paxman, Jason J" uniqKey="Paxman J" first="Jason J" last="Paxman">Jason J. Paxman</name><name sortKey="Subedi, Pramod" sort="Subedi, Pramod" uniqKey="Subedi P" first="Pramod" last="Subedi">Pramod Subedi</name><name sortKey="White, Jonathan M" sort="White, Jonathan M" uniqKey="White J" first="Jonathan M" last="White">Jonathan M. White</name><name sortKey="Wong, Wallace W H" sort="Wong, Wallace W H" uniqKey="Wong W" first="Wallace W H" last="Wong">Wallace W H. Wong</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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